Long-term preservation of biological samples is essential for reproducible research and future clinical use. Cryopreservation and biobanking support the continuity of regenerative medicine by storing viable cells, tissues, and bioengineered constructs without compromising their functionality. Innovations in cryoprotectants, controlled-rate freezing, and vitrification techniques now allow higher post-thaw survival rates and functionality of stem cells and engineered tissues. These advances are crucial for ensuring the availability of genetically matched materials for transplantation and personalized therapies. Cryopreservation and biobanking also play a central role in multi-center trials and biorepositories, enabling the sharing of standardized, quality-controlled samples. As demand increases for diverse and ethically sourced biospecimens, robust biobanking practices become a cornerstone of translational success in tissue engineering and regenerative medicine.