Effects of mechanical vibrations on growth and membrane stability of callus tissues in Hyoscyamus reticulates
Bahareh Sadat Haddadi is a PhD student at Aberystwyth University. She is focused on enhancing comprehension of tef, a gluten-free cereal, particularly in the face of environmental challenges like drought. Ms. Haddadi previously obtained a master’s degree from the University of Tehran in 2015 where she worked on the physiological and biochemical responses of Mentha aquatica to drought stress. Bahareh's research journey has been shaped by a passion for plant science and plant stress biology. Guided by Prof. Luis Mur, she is exploiting metabolomic and transcriptomic profiling of tef's responses to understand the responses of tef and other cereals to environmental stress.
Abstract:
Mechanical vibration is one of the abiotic stresses that can have effect on plant cells growth and development. Hyoscyamusreticulatesis a medicinal plant belonging to Solanaceae family. In this study, effects of mechanical vibration was evaluated on growth rate, total protein content and membrane damages of callus tissues in H. reticulatus. Mechanical vibration was applied on callus tissues in four levels (0, 50, 100, 150 Hz). Results indicated that mechanical vibration cause to increase of growth from control to 100 Hz, and then decreased at more than 100 Hz. Vibration (100 Hz) caused to increase of 60.6% dry weight in callus tissues compared to control. Total protein content increased under vibration and the highest protein content was observed in 50 Hz. Lipid peroxidation significantly decreased up to 100 Hz, and then increased with the increase of vibration level. It seems that mechanical vibration can be an appropriate treatment for growth increase of cells tissues and membrane stability of Hyoscyamus reticulatus.