The intersection of artificial intelligence, machine learning, and tissue science is reshaping how biological data is interpreted and applied. AI and big data in tissue engineering enable unprecedented levels of precision in designing scaffolds, selecting cell sources, and predicting regenerative outcomes. Through pattern recognition and algorithmic modeling, AI systems can process vast volumes of multi-omics and imaging data, revealing correlations invisible to human analysis. Researchers now use predictive analytics to simulate cellular behavior, refine bioprinting strategies, and anticipate immune responses to implants. By integrating clinical and preclinical data, AI-driven platforms are also helping accelerate patient stratification and personalized therapeutic design. AI and Big Data in Tissue Engineering represent not just technological advancement, but a paradigm shift in the way research is conducted and therapies are developed—providing a foundation for adaptive, data-informed regenerative solutions that continuously evolve based on real-world performance.