Dae Dong Sung | Korea University at Sejong Campus
Abstract
A simple gradient reversed-phase ultra-performance liquid chromatographic (RP-UPLC) method for the simultaneous determination of five pesticides, namely Monocrotophos, Thiram, Carbendazim, Carbaryl, and Imidacloprid has been developed. The aforementioned method involves sample preparation by QuEChERS method and quantification by ultra-performance liquid chromatography with the tunable dual wavelength detector. The mobile phase composition was varied to improve peak resolution and peak sensitivity. Choosing the match between the stationary phase and mobile phase composition, the developed RP-UPLC method not only can simplify the procedure but also significantly reduce the analysis time. The method presents an average recovery of more than 87.0% and 96.0%, in repeatability and intermediate precision conditions, respectively, with adequate precision (RSD from 0.8 to 20.5%), for all compounds. The method was applied to determine these pesticide residues in Mango and pomegranate samples.
